Journal: Experimental and Therapeutic Medicine

Article Title: Evaluation of the efficacy of Ajuga decumbens extract supplement in individuals with knee discomfort associated with physical activity: A randomized, double-blind, placebo-controlled study

doi: 10.3892/etm.2017.5064

Figure Lengend Snippet: Baseline characteristics of the study population in the ADE diet and placebo groups.

Article Snippet: Serum and urine samples were immediately used for routine laboratory tests; sera and urine samples were aliquoted and stored at −80°C until the assays of CTX-II, C2C, PIICP and MMP-13 using their respective ELISA assay kits: CTX-II, Urine CartiLaps EIA (AC-10F1, Immunodiagnostic Systems, Ltd., Tyne & Wear, UK); C2C, Collagen Type II Cleavage ELISA (60–1001-001; IBEX Technologies, Inc., Montreal, QC, Canada,); PIICP, Enzyme-linked Immunosorbent Assay kit For Procollagen II C-Terminal Propeptide (SEA964Hu; Uscn Life Sciences, Inc., Wuhan, China); and MMP-13, ELISA Kit For Matrix Metalloproteinase 13 (SEA099Hu; Uscn Life Sciences, Inc.).

Techniques:

Journal: Experimental and Therapeutic Medicine

Article Title: Evaluation of the efficacy of Ajuga decumbens extract supplement in individuals with knee discomfort associated with physical activity: A randomized, double-blind, placebo-controlled study

doi: 10.3892/etm.2017.5064

Figure Lengend Snippet: Levels of biomarkers for CII metabolism and the ratio of CII destruction to synthesis during the intervention period in the ADE diet (n=22) and placebo (n=22) groups.

Article Snippet: Serum and urine samples were immediately used for routine laboratory tests; sera and urine samples were aliquoted and stored at −80°C until the assays of CTX-II, C2C, PIICP and MMP-13 using their respective ELISA assay kits: CTX-II, Urine CartiLaps EIA (AC-10F1, Immunodiagnostic Systems, Ltd., Tyne & Wear, UK); C2C, Collagen Type II Cleavage ELISA (60–1001-001; IBEX Technologies, Inc., Montreal, QC, Canada,); PIICP, Enzyme-linked Immunosorbent Assay kit For Procollagen II C-Terminal Propeptide (SEA964Hu; Uscn Life Sciences, Inc., Wuhan, China); and MMP-13, ELISA Kit For Matrix Metalloproteinase 13 (SEA099Hu; Uscn Life Sciences, Inc.).

Techniques:

Journal: Experimental and Therapeutic Medicine

Article Title: Evaluation of the efficacy of Ajuga decumbens extract supplement in individuals with knee discomfort associated with physical activity: A randomized, double-blind, placebo-controlled study

doi: 10.3892/etm.2017.5064

Figure Lengend Snippet: Baseline characteristics of the subgroup participants with mild knee pain in the ADE diet and placebo groups.

Article Snippet: Serum and urine samples were immediately used for routine laboratory tests; sera and urine samples were aliquoted and stored at −80°C until the assays of CTX-II, C2C, PIICP and MMP-13 using their respective ELISA assay kits: CTX-II, Urine CartiLaps EIA (AC-10F1, Immunodiagnostic Systems, Ltd., Tyne & Wear, UK); C2C, Collagen Type II Cleavage ELISA (60–1001-001; IBEX Technologies, Inc., Montreal, QC, Canada,); PIICP, Enzyme-linked Immunosorbent Assay kit For Procollagen II C-Terminal Propeptide (SEA964Hu; Uscn Life Sciences, Inc., Wuhan, China); and MMP-13, ELISA Kit For Matrix Metalloproteinase 13 (SEA099Hu; Uscn Life Sciences, Inc.).

Techniques:

Journal: Biomedicines

Article Title: Cartilage Collagen Neoepitope C2C Expression in the Articular Cartilage and Its Relation to Joint Tissue Damage in Patients with Knee Osteoarthritis

doi: 10.3390/biomedicines12051063

Figure Lengend Snippet: Results of histopathology assessments and C2C staining of samples from 27 patients (10 male and 17 female patients; in each subject, two load-bearing sites of the tibial plateau were analyzed).

Article Snippet: One antibody specific to C2C is used in the Type II Collagen Cleavage Sandwich Assay developed by IBEX Pharmaceuticals Inc.

Techniques: Histopathology, Staining

Journal: Biomedicines

Article Title: Cartilage Collagen Neoepitope C2C Expression in the Articular Cartilage and Its Relation to Joint Tissue Damage in Patients with Knee Osteoarthritis

doi: 10.3390/biomedicines12051063

Figure Lengend Snippet: ( A ) Micrograph of a sample with histopathology grade 0: weak C2C immunostaining (brown) in the superficial and deep zones (counterstaining of proteoglycans with Toluidine-blue). The cartilage content of proteoglycans determined by the amount of Toluidine-blue staining was decreased in the tissue samples with more advanced OARSI tissue damage grade; ( B ) Micrograph of a sample with histopathology grade 1.5: moderate C2C immunostaining (brown) in the superficial and upper parts of the middle zone (counterstaining of proteoglycans with Toluidine-blue); ( C ) Micrograph of a sample with histopathology grade 3.5: overall abundant C2C immunostaining (brown), weak counterstaining in the deep zone (counterstaining of proteoglycans with Toluidine-blue); ( D ) Micrograph of a sample with histopathology grade 6: abundant C2C immunostaining (brown) in the remaining cartilage; subchondral bone, which occupies most of the specimen, is virtually not stained (counterstaining of proteoglycans with Toluidine-blue). A sample picture of negative control where the primary antibody was omitted is presented as an inset in the upper-right corner of micrograph ( D ). All micrographs were taken using a 5× objective.

Article Snippet: One antibody specific to C2C is used in the Type II Collagen Cleavage Sandwich Assay developed by IBEX Pharmaceuticals Inc.

Techniques: Histopathology, Immunostaining, Staining, Negative Control

Journal: The American Journal of Sports Medicine

Article Title: Association of Serum Biochemical Biomarker Profiles of Joint Tissue Inflammation and Cartilage Metabolism With Posttraumatic Osteoarthritis-Related Symptoms at 12 Months After ACLR

doi: 10.1177/03635465241262797

Figure Lengend Snippet: Concentrations of Serum Biochemical Biomarkers Between Profiles a

Article Snippet: Upon completion of the study, serum samples were batch processed in duplicate using commercial enzyme-linked immunosorbent assays to determine biomarker serum concentrations of MCP-1 (sMCP-1), COMP (sCOMP), and MMP-3 (sMMP-3) (R&D Systems) as well as C2C:CPII (sC2C:sCPII) ratio (IBEX Technologies).

Techniques: Biomarker Assay

Journal: Pharmaceutics

Article Title: Sustained Intra-Articular Release and Biocompatibility of Tacrolimus (FK506) Loaded Monospheres Composed of [PDLA-PEG 1000 ]- b -[PLLA] Multi-Block Copolymers in Healthy Horse Joints

doi: 10.3390/pharmaceutics13091438

Figure Lengend Snippet: Synovial fluid concentrations of markers for cartilage metabolism over time for all 4 treatment groups: glycosaminoglycans (GAGs) ( A ) and collagen II cleavage marker (C2C) ( B ). Graphs show the mean ± SD. Significant differences are depicted as follows: treatment groups vs. saline (*), unloaded vs. tacrolimus-loaded monospheres (#), or differences compared to the baseline within the same treatment group (a). One symbol: p > 0.01, two symbols: p < 0.01, three symbols: p < 0.001, and four symbols: p < 0.0001.

Article Snippet: To check for possible damage to the collagen network of the cartilage, we also measured the concentration of C2C, a neo-epitope present on collagenase-cleavage fragments of type II collagen, employing a commercial ELISA kit (IBEX Technologies, QC, Canada) in accordance with the manufacturer’s recommendations.

Techniques: Marker

Journal: Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society

Article Title: Loss of Extracellular Matrix from Articular Cartilage is Mediated by the Synovium and Ligament after Anterior Cruciate Ligament Injury

doi: 10.1016/j.joca.2013.09.003

Figure Lengend Snippet: Synovial fluid concentrations of C2C, CPII, C2C:CPII, and CS 846. The synovial fluid aspirated from unoperated knees at Day 0 serves as the baseline control. Data is reported as median values, with error bars representing 25th and 75th percentiles. Asterisks indicate significant differences from control at P<0.05 (n=3 per analysis).

Article Snippet: Collagen and Aggrecan Fragment Detection by ELISA Synovial fluid and serum C2C, CPII, CS 846 levels were determined using commercially-available ELISA kits (60-1001-001, 60-1004, and 60-1003-01; IBEX Pharmaceuticals Inc, Montreal, Canada) as previously described for porcine tissue ( 18 - 20 ).

Techniques: Control

Journal: Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society

Article Title: Loss of Extracellular Matrix from Articular Cartilage is Mediated by the Synovium and Ligament after Anterior Cruciate Ligament Injury

doi: 10.1016/j.joca.2013.09.003

Figure Lengend Snippet: Serum concentrations of C2C, CPII, C2C:CPII, and CS 846. The synovial fluid aspirated from unoperated knees at at Day 0 serves as the baseline control. Data is reported as median values, with error bars representing 25th and 75th percentiles. No statistical differences were found across days (n=6 per analysis).

Article Snippet: Collagen and Aggrecan Fragment Detection by ELISA Synovial fluid and serum C2C, CPII, CS 846 levels were determined using commercially-available ELISA kits (60-1001-001, 60-1004, and 60-1003-01; IBEX Pharmaceuticals Inc, Montreal, Canada) as previously described for porcine tissue ( 18 - 20 ).

Techniques: Control